Structural and mechanistic classification of uronic acid-containing polysaccharide lyases

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DOIResolve DOI: http://doi.org/10.1093/glycob/cwq122
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TypeArticle
Journal titleGlycobiology
Volume20
Issue12
Pages15471573; # of pages: 27
Subjectpolysaccharide lyases; glycosaminoglycans; classification; enzymatic mechanism; fold analysis
AbstractPolysaccharide lyases (PLs) have been assigned to 21 families based on their sequences, with ~50 singletons awaiting further classification. For 19 of these families, the structure of at least one protein is known. In this review, we have analyzed the available structural information and show that presently known PL families belong to six general folds. Only two general catalytic mechanisms have been observed among these PLs: (1) metal-assisted neutralization of the acidic group of the sugar next to the cleaved bond, with, rather unusually, arginine or lysine playing the role of Brønsted base and (2) neutralization of the acidic group on the sugar by a close approach of an amino or acidic group forcing its protonation and Tyr or Tyr-His acting as the Brønsted base and acid.
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedYes
NRC number53141
NPARC number17673437
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Record identifier8899cf95-74fa-40ce-8f57-15085cad602e
Record created2011-04-05
Record modified2016-05-09
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