PCR-free DNA detection using a magnetic bead-supported polymeric transducer and microelectromagnetic traps

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DOIResolve DOI: http://doi.org/10.1021/ac060486n
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TypeArticle
Journal titleAnalytical Chemistry
Volume78
Issue13
Pages44574464; # of pages: 8
AbstractA fluorescent polymeric hybridization transducer supported on magnetic microbeads was investigated for the rapid, ultrasensitive, and sequence-specific detection of DNA. We show that the polymer derivative can be used to detect target DNA directly on magnetic particles by preparing "target-ready" microbeads grafted wtih the polymer and suitable DNA probes. A detection limit of ~200 target copies in a probed volume of 150 µL (1.4 copies/µL) was obtained for a DNA sequence specific to Candida albicans. This detection scheme does not require the release of the hybridized target DNA prior to its detection or the labeling or amplification of the nucleic acids. Furthermore, we show that the fluorescence from these biosensing magnetic beads can be read while magnetically confined in a small volume by a microelectromagnetic trap, which offers the possibility of performing both the preconcentration and detection steps simultaneously on the same support. The combination of the fluorescent polymer biosensor with magnetic particle-assisted DNA preconcentration extends the application of this ultrasensitive biosensor to biological samples with complex matrixes and to integrated lab-on-a-chip platforms, where it could be used for fast multitarget DNA detection in point-of-care diagnostics and field analysis.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Industrial Materials Institute
Peer reviewedYes
NRC number48926
NPARC number15884124
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Record identifier8928f921-ee24-418f-91dd-f3f204f4050b
Record created2010-07-30
Record modified2016-05-09
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