Intracellular imaging of HCV RNA and cellular lipids by using simultaneous two-photon fluorescence and coherent anti-stokes Raman scattering microscopies

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DOIResolve DOI: http://doi.org/10.1002/cbic.200600330
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TypeArticle
Journal titleChemBioChem
Volume7
Issue12
Pages18951897; # of pages: 3
SubjectCARS microscopy; hepatitis C virus; host–viral interactions; RNA; two-photon fluorescence
AbstractHepatitis C virus (HCV) infection is associated with changes in host-cell lipid metabolism. Here we describe a new approach for detecting HCV RNA using two-photon fluorescence (TPF), and HCV-associated changes in cellular lipids using coherent anti-Stokes Raman scattering (CARS) microscopy. By combining the two types of microscopy with a common laser source, we visualized both phenomena simultaneously and profiled cellular lipids and subcellular localization of RNA in real time.
Publication date
AffiliationNational Research Council Canada; NRC Steacie Institute for Molecular Sciences
Peer reviewedYes
NPARC number12328952
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Record identifier8aff03c6-aa10-4edd-889f-ddcee17a9b6c
Record created2009-09-10
Record modified2016-05-09
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