Rainbow smelt (Osmerus mordax) genomic library and EST resources

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DOIResolve DOI: http://doi.org/10.1007/s10126-008-9089-6
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TypeArticle
Journal titleMarine Biotechnology
Volume10
Issue5
Pages487491; # of pages: 5
AbstractGenomic resources in rainbow smelt (Osmerus mordax) enable us to examine the genome duplication process in salmonids and test hypotheses relating to the fate of duplicated genes. They further enable us to pursue physiological and ecological studies in smelt. A bacterial artificial chromosome library containing 52,410 clones with an average insert size of 146 kb was constructed. This library represents an 11-fold average coverage of the rainbow smelt (O. mordax) genome. In addition, several complementary deoxyribonucleic acid libraries were constructed, and 36,758 sequences were obtained and combined into 12,159 transcripts. Over half of these transcripts have been identified, several of which have been associated with cold adaptation. These basic resources show high levels of similarity (86%) to salmonid genes and provide initial support for genome duplication in the salmonid ancestor. They also facilitate identification of genes important to fish and direct us toward new technologies for other studies in fish biology.
Publication date
PublisherSpringer International Publishing Ag
LanguageEnglish
AffiliationNRC Institute for Marine Biosciences; National Research Council Canada; Aquatic and Crop Resource Development
Peer reviewedYes
NRC number42762
1725
NPARC number3538520
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Record identifier8c4cc8ba-4e20-4a8f-94e4-36528f34a1f9
Record created2009-03-01
Record modified2016-05-09
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