Toxic cyanobacterial bloom triggers in missisquoi bay, lake champlain, as determined by next-generation sequencing and quantitative PCR

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DOIResolve DOI: http://doi.org/10.3390/life5021346
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TypeArticle
Journal titleLife
ISSN2075-1729
Volume5
Issue2
Pages13461380; # of pages: 35
SubjectChroococcales; Cyanobacteria; Microcystis; Nostocales
AbstractMissisquoi Bay (MB) is a temperate eutrophic freshwater lake that frequently experiences toxic Microcystis-dominated cyanobacterial blooms. Non-point sources are responsible for the high concentrations of phosphorus and nitrogen in the bay. This study combined data from environmental parameters, E. coli counts, high-throughput sequencing of 16S rRNA gene amplicons, quantitative PCR (16S rRNA and mcyD genes) and toxin analyses to identify the main bloom-promoting factors. In 2009, nutrient concentrations correlated with E. coli counts, abundance of total cyanobacterial cells, Microcystis 16S rRNA and mcyD genes and intracellular microcystin. Total and dissolved phosphorus also correlated significantly with rainfall. The major cyanobacterial taxa were members of the orders Chroococcales and Nostocales. The genus Microcystis was the main mcyD-carrier and main microcystin producer. Our results suggested that increasing nutrient concentrations and total nitrogen:total phosphorus (TN:TP) ratios approaching 11:1, coupled with an increase in temperature, promoted Microcystis-dominated toxic blooms. Although the importance of nutrient ratios and absolute concentrations on cyanobacterial and Microcystis dynamics have been documented in other laboratories, an optimum TN:TP ratio for Microcystis dominance has not been previously observed in situ. This observation provides further support that nutrient ratios are an important determinant of species composition in natural phytoplankton assemblages.
Publication date
PublisherMDPI AG
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); Energy, Mining and Environment
Peer reviewedYes
NPARC number21275646
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Record identifier8e66978b-c801-4ce3-9d9c-b819fb0b1021
Record created2015-07-14
Record modified2016-05-09
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