Widespread occurrence of chromosomal aneuploidy following the routine production of Candida albicans mutants

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DOIResolve DOI: http://doi.org/10.1111/j.1567-1364.2009.00563.x
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TypeArticle
Journal titleFEMS Yeast Research
Volume9
Issue7
Pages10701077; # of pages: 8
SubjectAnnotation; bio,crossector; Biotechnology; Candida; Candida albicans; Cells; Chromosome structure; Chromosomes; Cyclic AMP; env; Gene Deletion; genetics; genome; Genome sequence; Genomics; Human; Karyotype; methods; Microarray; Mutation; pathogenicity; Virulence; Yeast; Yeasts; Zinc
AbstractIt has come to our attention that approximately 35% of >100 published micro-array data sets, where transcript levels were compared between two different strains, exhibit some form of chromosome-specific bias. While some of these arose from the use of strains whose aneuploidies were not known at the time, in a worrisome number of cases the recombinant strains have acquired additional aneuploidies that were not initially present in the parental strain. The aneuploidies often affected a different chromosome than the one harboring the insertion site. The affected strains originated from either CAI-4, RM1000, BWP17 or SN95 and were produced through a variety of strategies. These observations suggest that aneuploidies frequently occur during the production of recombinant strains and have an effect on global transcript profiles outside of the afflicted chromosome(s), thus raising the possibility of unintended phenotypic consequences. Thus, we propose that all Candida albicans mutants and strains should be tested for aneuploidy before being used in further studies. To this end, we describe a new rapid testing method, based on a multiplex quantitative PCR assay, that produces eight bands of distinct sizes from either the left or right arms of each C. albicans chromosome.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedYes
NRC number50662
NPARC number12905907
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Record identifier8eef1a2c-8b0f-4909-8157-74052ed77484
Record created2009-11-10
Record modified2016-05-09
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