Optimum extraction of polysaccharides from Opuntia dillenii and evaluation of its antioxidant activities

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DOIResolve DOI: http://doi.org/10.1016/j.carbpol.2013.05.057
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TypeArticle
Journal titleCarbohydrate Polymers
ISSN0144-8617
Volume97
Issue2
Pages736742; # of pages: 7
SubjectAnti-oxidant activities; Antioxidant capacity; Box-Behnken design; Extraction conditions; Extraction temperatures; Extraction time; Opuntia dillenii; Response surface methodology; Agents; Optimization; Polysaccharides; Surface properties; Extraction; Opuntia; Opuntia dillenii
AbstractResponse surface methodology (RSM) was applied to optimize the extraction of crude polysaccharides from Opuntia dillenii (Ker-Gaw) Haw. A three-level, four-variable Box-Behnken design was employed to obtain the best possible combination of extraction temperature (80-90°C), extraction time (50-70 min), number of extraction cycle (1-3 times), and ratio of water to raw material (8:1-12:1, v/w) for maximum yield of crude polysaccharide. Besides, the antioxidant capacity of crude polysaccharide was evaluated by DPPH assay. The results showed that optimized extraction conditions were extraction temperature 85°C, extraction time 63.7 min, extraction 2 times and ratio of water to raw material 11.14:1. Under these conditions, the experimental yield was 27.36 ± 0.21%, which is well in close agreement with the value (27.44%) predicted by RSM model. Pharmacological test showed that O. dillenii crude polysaccharides had a good antioxidant activity. © 2013 Elsevier Ltd. All rights reserved.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Nutrisciences and Health (INH-ISNS)
Peer reviewedYes
NPARC number21269901
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Record identifier8fc6d886-bbaf-4e31-ae5f-b82822ef77e7
Record created2013-12-13
Record modified2016-05-09
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