Nanocomposites of nanocrystalline cellulose for enzyme immobilization

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Journal titleCellulose
Pages191200; # of pages: 10
SubjectAcid groups; Biosensing applications; Carbodiimides; Carbon chains; Cationic polyethylenimine; Chemical affinities; Gold nanoparticle; Metallic nanoparticles; Nanocrystalline cellulose; Natural cellulose fibers; Renewable sources; Surface area; Biosensors; Carbon; Carboxylic acids; Cellulose derivatives; Glucose sensors; Gold; Metal nanoparticles; Nanocomposites; Synthesis (chemical); Enzyme immobilization; Carboxylic Acids; Cellulose Derivatives; Composites; Crystallites; Gold Compounds; Sensors; Synthesis
AbstractWe describe the synthesis, characterization and use of a composite material made of a renewable source and metallic nanoparticles for biosensing applications. Nanocrystalline cellulose (NCC) is a product isolated from natural cellulose fibers, which is of approximately 100 nm long and 10 nm wide in size. We augmented the surface area and tailored the chemical affinity of NCC by optimally dressing it with gold nanoparticles (AuNPs). The deposition of AuNPs on NCC was controlled by using cationic polyethylenimine (PEI) at different pHs. AuNPs were thiol-functionalized using different linkers prior to enzyme immobilization. The enzyme (glucose oxidase or GOx) was conjugated on the composite by carbodiimide coupling, and subsequent activation of linker-carboxylic acid group. Our results showed that GOx was attached to the surface of the NCC nanocomposite. Moreover, the amount of GOx loaded onto the support depended on the length of the thiol-linker used. The lower value (20. 3 mg/mg of support) was obtained with the longer thiol-linker (11 carbon chain) compared to 25. 2 mg/mg of support for the smaller thiol-linker (3 carbon chain). © 2012 Springer Science+Business Media Dordrecht.
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AffiliationNational Research Council Canada (NRC-CNRC); National Institute for Nanotechnology (NINT-INNT)
Peer reviewedYes
NPARC number21269753
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Record identifier905d30f0-dff0-424f-978b-b06e0f0ac257
Record created2013-12-13
Record modified2016-05-09
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