A ¹H-NMR method for determining temperature in cell culture perfusion systems

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DOIResolve DOI: http://doi.org/10.1002/mrm.1910290120
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TypeArticle
Journal titleMagnetic Resonance in Medicine
Volume29
Issue1
Pages113118; # of pages: 6
Subject1H-NMR; temperature; cell culture; perfusion
AbstractThis report describes a noninvasive 1H-NMR method for measuring absolute temperatures (±0.2°C) in biological samples and, in particular, in cell culture perfusion systems, utilizing the linear temperature dependence of the water chemical shift relative to the temperature-independent shift of one of the components of the biological medium, e.g., pyruvate, acetate, or lactate. The effects of flow on temperature can be monitored and appropriate adjustments of the temperature controller can be made.
Publication date
LanguageEnglish
Peer reviewedYes
NRC publication
This is a non-NRC publication

"Non-NRC publications" are publications authored by NRC employees prior to their employment by NRC.

NRC number377
NPARC number9742172
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Record identifier9090a8f6-5231-41a9-8abd-5d17b45b6514
Record created2009-07-17
Record modified2016-05-09
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