Purification, functionalization, and bioconjugation of carbon nanotubes.

  1. Get@NRC: Purification, functionalization, and bioconjugation of carbon nanotubes. (Opens in a new window)
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Journal titleMethods in molecular biology (Clifton, N.J.)
Pages505532; # of pages: 28
Subjectborane derivative; carbon nanotube; carboxylic acid; ferric ion; ferric oxide; gold; immobilized protein; ionic liquid; metal nanoparticle; nitric acid; polyethyleneimine; polymer; pyrene; pyrene derivative; silicon; styrenesulfonic acid polymer; sulfonic acid derivative; sulfuric acid; water; article; chemistry; mechanics; oxidation reduction reaction; solubility; static electricity; Borohydrides; Carboxylic Acids; Ferric Compounds; Gold; Immobilized Proteins; Ionic Liquids; Mechanical Processes; Metal Nanoparticles; Nanotubes, Carbon; Nitric Acid; Oxidation-Reduction; Polyethyleneimine; Polymers; Pyrenes; Silicon; Solubility; Static Electricity; Sulfonic Acids; Sulfuric Acids; Water
AbstractBioconjugation of carbon nanotubes (CNTs) with biomolecules promises exciting applications such as biosensing, nanobiocomposite formulation, design of drug vector systems, and probing protein interactions. Pristine CNTs, however, are virtually water-insoluble and difficult to evenly disperse in a liquid matrix. Therefore, it is necessary to attach molecules or functional groups to their sidewalls to enable bioconjugation. Both noncovalent and covalent procedures can be used to conjugate CNTs with a target biomolecule for a specific bioapplication. This chapter presents a few selected protocols that can be performed at any wet chemistry laboratory to purify and biofunctionalize CNTs. The preparation of CNTs modified with metallic nanoparticles, especially gold, is also described since biomolecules can bind and self-organize on the surfaces of such metal-decorated CNTs.
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AffiliationNational Research Council Canada (NRC-CNRC); NRC Biotechnology Research Institute (BRI-IRB)
Peer reviewedYes
NPARC number21271041
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Record identifier90ae1f8a-5d62-49a0-aeb2-fb5e200a9125
Record created2014-03-24
Record modified2016-05-09
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