Smart gold nanoparticles enhance killing effect on cancer cells

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DOIResolve DOI: http://doi.org/10.3892/ijo.2012.1721
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TypeArticle
Journal titleInternational Journal of Oncology
ISSN1019-6439
Volume42
Issue2
Pages597608; # of pages: 12
Subjectglucose capped gold nanoparticle; radiosensitizing agent; unclassified drug; antineoplastic activity; apoptosis; cancer radiotherapy; cell killing; cellular distribution; comparative study; controlled study; cytoplasm; drug effect; drug uptake; endocytosis; flow cytometry; human cell; internalization; nick end labeling; radiosensitization; transmission electron microscopy; X irradiation
AbstractThe present study explored the cellular uptake dynamics, the subcellular location and the internalization mechanisms of gold nanoparticles (GNPs) and glucose-capped GNPs (Glu-GNPs). The cancer radiotherapy-enhancing effects of GNPs were also evaluated. We synthesized the GNPs and Glu-GNPs by the seeding technique. The effects on cellular uptake and the radiosensitizing effect induced by GNPs and Glu-GNPs at lower doses were investigated using two human cancer cell lines (HeLa and MCF-7). The intracellular location of the nanoparticles was analyzed by transmission electron microscopy (TEM). Analysis of cellular apoptosis following GNP-based radiotherapy was performed by flow cytometry and TUNEL assay. Cancer cells took up more Glu-GNPs than naked GNPs and the uptake curve showed size- and cell-dependent uptake. GNPs were mainly located in the cytoplasm and endocytosis is the mechanism behind the internalization of GNPs and Glu-GNPs. Lower doses of GNPs and Glu-GNPs still enhanced the killing effect using X-ray irradiation, although the apoptotic rate was not altered. The results presented in this study provide evidence that Glu-GNPs may have a bright future in tumor-targeted diagnosis and treatment.
Publication date
LanguageEnglish
AffiliationSecurity and Disruptive Technologies; Human Health Therapeutics; National Research Council Canada
Peer reviewedYes
NPARC number21270458
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Record identifier9125de9d-04b0-49e1-b9f1-cf62b9b38f90
Record created2014-02-12
Record modified2016-05-09
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