Optimum levels of sulphate and iron for the cultivation of pure cultures of methanogens in synthetic media

Download
  1. Get@NRC: Optimum levels of sulphate and iron for the cultivation of pure cultures of methanogens in synthetic media (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1111/j.1365-2672.1978.tb04235.x
AuthorSearch for: ; Search for: ; Search for:
TypeArticle
Journal titleJournal of Applied Microbiology
Volume45
Issue3
Pages347356; # of pages: 10
AbstractExperiments on the effects, of sulphate and iron content of medium on the growth of Methanobacterium strain M.O.H., Methanospirllum hungatii GPI, and an unidentified strain similar in characteristics to Methanobacterium formicicum, indicated that the 4.0 mM of sulphate normally incorporated into laboratory media was above the optimum level, while the iron content normally incorporated (0.01–0.08 mM) was below the optimum requirement of these methanogens. The tests were made in synthetic media containing acetate, carbon dioxide and hydrogen as carbon and energy sources, and a cysteine-sodium sulphide solution as a reducing agent. The optimum sulphate requirement for Methanobacterium strain M.O.H., M. hungatii, and the unidentified methanogen was in the range of 0.16–0.52 mM. An absence of sulphate in the medium decreased significantly (95% confidence level) the growth of these cultures. The optimum requirement of Fe++ was in the range of 0.3–0.9 mM. Addition of Fe+++ greater than 0.10 mM to pre-reduced medium, or the addition of greater than 0.15 mM during preparation of medium resulted in precipitation of iron. A method for the preparation of medium without formation of precipitate is described and its contribution to improving growth and methanogenesis discussed.
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberPATEL1978
16602
NPARC number9388167
Export citationExport as RIS
Report a correctionReport a correction
Record identifier92f371e7-cd92-4eea-8912-121c78789a5b
Record created2009-07-10
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)