Laser ablation electrospray ionization-high resolution mass spectrometry for regulatory screening of domoic acid in shellfish

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DOIResolve DOI: http://doi.org/10.1002/rcm.7725
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TypeArticle
Journal titleRapid Communications in Mass Spectrometry
ISSN0951-4198
1097-0231
Volume30
Issue22
Pages23792446
AbstractRationale: Domoic acid (DA) is a potent neurotoxin that accumulates in shellfish. Routine testing involves homogenization, extraction and chromatographic analysis, with a run time of up to 30 min. Improving throughput using ambient ionization for direct analysis of DA in tissue would result in significant time savings for regulatory testing labs. Methods: We assess the suitability of laser ablation electrospray ionization high-resolution mass spectrometry (LAESI-HRMS) for high-throughput screening or quantitation of DA in a variety of shellfish matrices. The method was first optimized for use with HRMS detection. Challenges such as tissue sub-sampling, isobaric interferences and method calibration were considered and practical solutions developed. Samples included 189 real shellfish samples previously analyzed by regulatory labs as well as mussel matrix certified reference materials. Results: Domoic acid was selectively analyzed directly from shellfish tissue homogenates with a run time of 12 s. The limits of detection were between 0.24 and 1.6 mg DA kg−1 tissue, similar to those of LC/UV methods. The precision was between 27 and 44% relative standard deviation (RSD), making the technique more suited to screening than direct quantitation. LAESI-MS showed good agreement with LC/UV and LC/MS and was capable of identifying samples above and below 5 mg DA kg−1 wet shellfish tissue, one quarter of the regulatory limit. Conclusions: These findings demonstrate the suitability of LAESI-MS for routine, high-throughput screening of DA. This approach could result in significant time savings for regulatory labs carrying out shellfish safety testing on thousands of samples annually.
Publication date
PublisherWiley
LanguageEnglish
AffiliationNational Research Council Canada; Measurement Science and Standards
Peer reviewedYes
NPARC number23002013
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Record identifier93594999-e608-49d5-8692-7433e16bce99
Record created2017-07-20
Record modified2017-07-20
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