A modified fractionation procedure for avian erythrocyte histones

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DOIResolve DOI: http://doi.org/10.1139/o69-180
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TypeArticle
Journal titleCanadian Journal of Biochemistry
ISSN0008-4018
Volume47
Issue12
Pages11211123; # of pages: 3
AbstractThe fractionation method of Vidali and Neelin for avian erythrocyte histones was modified to reduce the time required to obtain clean fractions of serine-rich and arginine-rich histones. Histones were extracted from washed nuclei in one step with 0.20 M HCI. The lysine-rich and the moderately lysine-rich histones were fractionated by cation-exchange chromatography but the serine-rich and arginine-rich histones were eluted together. These histones were separated by subsequent exclusion chromatography.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada
Peer reviewedYes
NRC number10983
NPARC number21274594
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Record identifier93abf2ba-f997-4877-93b9-bf15e770e5b4
Record created2015-03-18
Record modified2016-05-09
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