State-of-the art comparability of corrected emission spectra. 2. Field laboratory assessment of calibration performance using spectral fluorescence standards

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DOIResolve DOI: http://doi.org/10.1021/ac203451g
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TypeArticle
Journal titleAnalytical Chemistry
Volume84
Issue9
Pages38993907; # of pages: 8
AbstractIn the second part of this two-part series on the state-of-the-art comparability of corrected emission spectra, we have extended this assessment to the broader community of fluorescence spectroscopists by involving 12 field laboratories that were randomly selected on the basis of their fluorescence measuring equipment. These laboratories performed a reference material (RM)- based fluorometer calibration with commercially available spectral fluorescence standards following a standard operating procedure that involved routine measurement conditions and the data evaluation software LINKCORR developed and provided by the Federal Institute for Materials Research and Testing (BAM). This instrument-specific emission correction curve was subsequently used for the determination of the corrected emission spectra of three test dyes, X, QS, and Y, revealing an average accuracy of 6.8% for the corrected emission spectra. This compares well with the relative standard uncertainties of 4.2% for physical standard-based spectral corrections demonstrated in the first part of this study (previous paper in this issue) involving an international group of four expert laboratories. The excellent comparability of the measurements of the field laboratories also demonstrates the effectiveness of RM-based correction procedures.
Publication date
PublisherAmerican Chemical Society
LanguageEnglish
AffiliationMeasurement Science and Standards; NRC Institute for National Measurement Standards; National Research Council Canada
Peer reviewedYes
NPARC number21268134
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Record identifier941f8580-4088-4531-98fc-faeddd43ea2a
Record created2013-05-08
Record modified2016-05-09
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