Microarray analysis identifies pathways in progression of early stage lung adenocarcinoma: the importance of focal adhesion and ECM-receptor interactions

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DOIResolve DOI: http://doi.org/10.17140/PRRMOJ-1-104
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TypeArticle
Journal titlePulmonary Research and Respiratory Medicine
ISSN2377-1658
Volume1
Issue1
Pages2131; # of pages: 11
SubjectAdenocarcinoma; Biomarkers; Microarray; Non-small cell lung cancer; Recurrence; Transcriptome
AbstractRecurrence after lung cancer surgery is high, even among Non-Small Cell Lung Cancer (NSCLC) adenocarcinoma patients diagnosed early as Stage I, where there has been no spread to lymph nodes. Understanding the biological underpinnings of aggressivity and recurrence in this subset of tumours may enable the identification of patients who would benefit from adjuvant therapy. The purpose of this study was to identify differentially expressed molecular biomarkers that might underlie recurrence of Stage I tumours by comparing gene expression in later-stage tumours with those expressed in early-stage tumours. Gene expression in tissue biopsy samples from five Stage I and five Stage II/III NSCLC adenocarcinoma patients was analysed using an oligonucleotide microarray containing 17,000 probes printed in duplicate. Analyses were performed on total RNA isolated from tumour tissue of each patient using universal human RNA as a reference. Compared to normal tissues, the transcriptome of Stage I NSCLC adenocarcinomas showed enrichment in general pathways in cancer, whereas in Stage II/III more specific cancer pathways such as focal adhesion and ECM-receptor interaction pathways were enriched and components of the PPAR signalling pathway were depleted. Relative to early-stage NSCLC, Stage II/III adenocarcinomas showed up-regulation of genes of the basic transcriptional and translational machinery, particularly the “cancer testis antigen” PASD1 transcription factor. The actin cytoskeleton re-organisation and interleukin-6 pathways were also up-regulated whereas there was a generalized down-regulation of immune effectors and genes involved in immune system development. This small-scale transcriptome study provides important information about the pathways and molecules likely to be involved in the more metastatic propensity of those Stage I NSCLC adenocarcinomas that recur.
Publication date
PublisherOpenventio
LanguageEnglish
AffiliationHuman Health Therapeutics; National Research Council Canada
Peer reviewedYes
NRC numberNRC-HHT-53285
NPARC number21275375
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Record identifier96a5364f-7d9d-44fc-ad13-771a7976b12d
Record created2015-06-17
Record modified2016-05-09
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