Similarities between the sensitivity to 2-chlorodeoxyadenosine of lymphocytes from CLL patients and bryostatin 1-treated WSU-CLL cells : an infrared spectroscopic study

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DOIResolve DOI: http://doi.org/10.1016/S0304-3835(98)00036-6
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TypeArticle
Journal titleCancer Letters
Volume127
Issue1-2
Pages185193; # of pages: 9
SubjectChronic lymphocytic leukemia; Bryostatin 1; 2-Chlorodeoxyadenosine; Drug resistance; Infrared spectroscopy
AbstractInfrared (IR) spectroscopy was used to compare the drug resistance mechanism of cells from chronic lymphocytic leukemia (CLL) patients with that of WSU-CLL cells. Bryostatin 1 (Bryo 1), a macrocyclic lactone and protein kinase C activator, was used to render WSU-CLL cells more susceptible to 2-chlorodeoxyadenosine (2-CdA). The IR spectroscopic analysis revealed some changes in protein and DNA content in Bryo 1-treated WSU-CLL cells, however, the most significant alterations were observed in the membrane lipids, which resemble those found between 2-CdA-sensitive and 2-CdA-resistant cells from CLL patients. In addition, Bryo 1 treatment induced WSU-CLL cells to become CD11c, CD25 and tartrate-resistant acid phosphatase-positive, specific markers for hairy cell leukemia, a disease exquisitely sensitive to 2-CdA. Our results suggest that 2-CdA-sensitive CLL cells have cellular characteristics resembling the hairy cell stage. The similarity between the membrane lipids in 2-CdA-sensitive CLL cells and the Bryo 1-treated WSU-CLL cell line supports the suggestion that membrane lipid alteration might be an important step in the drug resistance mechanism of CLL cells.
Publication date
PublisherElsevier
Copyright noticeYou may reproduce (print, make photocopies, or download) materials from NPArC without permission for non-commercial purposes (research, education, and private study), on the condition that you provide proper attribution of the sources in all copies.
LanguageEnglish
AffiliationNRC Institute for Biodiagnostics (IBD-IBD); National Research Council Canada
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public
Peer reviewedYes
IdentifierS0304-3835(98)00036-6
NPARC number9201847
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Record identifier994b9d1c-aed5-46a0-b87e-189208238e97
Record created2009-10-03
Record modified2016-10-18
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