Enhancing the detection of Giardia duodenalis cysts in foods by inertial microfluidic separation

  1. Get@NRC: Enhancing the detection of Giardia duodenalis cysts in foods by inertial microfluidic separation (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1128/AEM.03868-14
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Journal titleApplied and Environmental Microbiology
Pages39253933; # of pages: 9
SubjectFluidic devices; Microfluidics; Protozoa; Concentration method; Conventional methods; Immunofluorescence microscopy; Inertial microfluidics; Limit of detection; Low concentrations; Micro-fluidic devices; Sensitivity and specificity; Suspensions (fluids); biotechnology; concentration (composition); cyst; food; protozoan; Giardia; Giardia intestinalis; Lactuca
AbstractThe sensitivity and specificity of current Giardia cyst detection methods for foods are largely determined by the effectiveness of the elution, separation, and concentration methods used. The aim of these methods is to produce a final suspension with an adequate concentration of Giardia cysts for detection and a low concentration of interfering food debris. In the present study, a microfluidic device, which makes use of inertial separation, was designed and fabricated for the separation of Giardia cysts. A cyclical pumping platform and protocol was developed to concentrate 10-ml suspensions down to less than 1 ml. Tests involving Giardia duodenalis cysts and 1.90-μm microbeads in pure suspensions demonstrated the specificity of the microfluidic chip for cysts over smaller nonspecific particles. As the suspension cycled through the chip, a large number of beads were removed (70%) and the majority of the cysts were concentrated (82%). Subsequently, the microfluidic inertial separation chip was integrated into a method for the detection of G. duodenalis cysts from lettuce samples. The method greatly reduced the concentration of background debris in the final suspensions (10-fold reduction) in comparison to that obtained by a conventional method. The method also recovered an average of 68.4% of cysts from 25-g lettuce samples and had a limit of detection (LOD) of 38 cysts. While the recovery of cysts by inertial separation was slightly lower, and the LOD slightly higher, than with the conventional method, the sample analysis time was greatly reduced, as there were far fewer background food particles interfering with the detection of cysts by immunofluorescence microscopy.
Publication date
PublisherAmerican Society for Microbiology
AffiliationNational Research Council Canada; Medical Devices
Peer reviewedYes
NPARC number21275839
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Record identifier99f26c88-238a-4686-8cca-4a7d49f1e0a5
Record created2015-07-14
Record modified2016-05-09
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