Calnexin-dependent regulation of tunicamycin-induced apoptosis in breast carcinoma MCF-7 cells

Download
  1. Get@NRC: Calnexin-dependent regulation of tunicamycin-induced apoptosis in breast carcinoma MCF-7 cells (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1038/sj.cdd.4402012
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleCell death and differentiation
ISSN1350-9047
Volume14
Issue3
Pages586596; # of pages: 11
SubjectAmino Acid Sequence; Apoptosis; Bap31; Breast Neoplasms; Calnexin; Carcinoma; Caspase 3; Cell Line, Tumor; Clone Cells; Drug Resistance, Neoplasm; Endoplasmic Reticulum; Enzyme Activation; ER stress; Gene Expression Regulation, Neoplastic; Humans; Molecular Sequence Data; Tunic amycin
AbstractThe endoplasmic reticulum (ER) has evolved specific mechanisms to ensure protein folding as well as the maintenance of its own homeostasis. When these functions are not achieved, specific ER stress signals are triggered to activate either adaptive or apoptotic responses. Here, we demonstrate that MCF-7 cells are resistant to tunicamycin-induced apoptosis. We show that the expression level of the ER chaperone calnexin can directly influence tunicamycin sensitivity in this cell line. Interestingly, the expression of a calnexin lacking the chaperone domain (DeltaE) partially restores their sensitivity to tunicamycin-induced apoptosis. Indeed, we show that DeltaE acts as a scaffold molecule to allow the cleavage of Bap31 and thus generate the proapoptotic p20 fragment. Utilizing the ability of MCF-7 cells to resist tunicamycin-induced apoptosis, we have characterized a molecular mechanism by which calnexin regulates ER-stress-mediated apoptosis in a manner independent of its chaperone functions but dependent of its binding to Bap31.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
Identifier1053054x
NPARC number12330241
Export citationExport as RIS
Report a correctionReport a correction
Record identifier9aaa67b4-6a94-4b35-9d41-ad97d9c33a9c
Record created2009-09-10
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)