Colonization of gerbils with Helicobacter pylori O-chain-deficient mutant SS1 HP0826:: Kan results in gastritis and is associated with de novo synthesis of extended homopolymers

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DOIResolve DOI: http://doi.org/10.1111/2049-632X.12021
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TypeArticle
Journal titlePathogens and Disease
ISSN2049-632X
Volume67
Issue2
Pages9199; # of pages: 9
Subjectbacterium lipopolysaccharide; galactan; O antigen; adaptation; animal experiment; animal model; animal tissue; article; bacterial colonization; bacterial strain; bacterium isolation; bacterium mutant; cell infiltration; cell loss; chronic gastritis; controlled study; disease association; gerbil; Helicobacter infection; Helicobacter pylori; histopathology; inoculation; male; metaplasia; methylation; nonhuman; polyacrylamide gel electrophoresis; polymorphonuclear cell; priority journal; stomach atrophy; stomach parietal cell; wild type; Animals; Carbohydrate Sequence; Electrophoresis, Polyacrylamide Gel; Gastric Mucosa; Gastritis; Gerbillinae; Helicobacter Infections; Helicobacter pylori; Male; Mutation; O Antigens; Stomach
AbstractThe O-chain polysaccharide of Helicobacter pylori is important for colonization and generation of chronic gastritis in mice. There are marked host differences in the development of H. pylori-induced gastric pathology in mice and gerbils. To investigate the role of the O-chain polysaccharide of H. pylori in colonization and gastritis in Mongolian gerbils, inoculation by oral gavage with H. pylori strain SS1 and its corresponding O-chain polysaccharide-deficient mutant SS1 HP0826::Kan was undertaken. Infection with both strains resulted in corpus atrophy, loss of parietal cells, and extensive mucous metaplasia at both 18 and 30 weeks postinfection. Contrary to previous results in splenocyte recipient severe combined immunodeficiency (SCID) mice, no difference was found in the grade of chronic gastritis, polymorphonuclear cell infiltration, atrophy, and mucous metaplasia in gerbils infected with the wild-type SS1 strain or SS1 HP0826::Kan strain. Examination of the effects of gerbil passage on LPS profiles of output SS1 HP0826::Kan isolates by SDS-PAGE, sugar, and methylation analyses revealed significant differences in LPS profiles of SS1 HP0826::Kan cells recovered from infected gerbils as compared to input bacteria. Specifically, the presence of a novel homopolymer of d-galactan, as well as an extended polymer of d-riban, was detected. These data provide evidence for the role of H. pylori LPS in bacterial adaption to promote colonization and pathology. © 2013 Federation of European Microbiological Societies.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC)
Peer reviewedYes
NPARC number21270684
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Record identifier9b05c5b2-5978-420b-aa29-221d04936458
Record created2014-02-17
Record modified2016-05-09
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