Derivatization, stabilization and detection of biogenic amines by cyclodextrin-modified capillary electrophoresis-laser-induced fluorescence detection

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DOIResolve DOI: http://doi.org/10.1016/S0021-9673(01)01056-1
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TypeArticle
Journal titleJournal of Chromatography A
Volume926
Issue2
Pages309317; # of pages: 9
SubjectDerivatization, electrophoresis; Food analysis
Abstracto-Phthalaldehyde (OPA) derivatives of eight biogenic amines were stabilized at 5°C by forming inclusion complexes with methyl-β-cyclodextrin (MBCD). The derivatives were separated and detected by cyclodextrin-modified capillary electrophoresis (CE) with UV or laser-induced fluorescence (LIF) detection. Using a borate buffer, pH 9.0 consisting of ethanol and a mixture of negatively charged sulfobutylether-β-cyclodextrin and neutral MBCD, baseline separation of the eight OPA derivatives was achieved within 25 min with high separation efficiencies. The detection limits (S/N=3) obtained by UV and LIF detection were determined to be 10 μM and 0.250 μM, respectively. Glutamic acid was added after the initial derivatization step to neutralize residual OPA which otherwise caused a significant interference, particularly when analysis was performed around the detection limit of the OPA derivatives. Important biogenic amines in fish, wine and urine were then derivatized and determined by CE–LIF. In the case of sole and rainbow trout, the results obtained were validated by an enzymatic assay using putrescine oxidase.
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AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number44633
NPARC number3539872
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Record identifier9c6b4bea-35f5-4eb5-97d6-fd394affea72
Record created2009-03-01
Record modified2016-05-09
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