Structural determination of the capsular polysaccharide of Neisseria meningitidis group I: a two-dimensional NMR analysis: Biochemistry

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TypeArticle
Journal titleBiochemistry
Volume24
Issue20
Pages55925598; # of pages: 7
Subjectanalysis; anisotropy; ANOMERIC; Antibodies; antibody; ANTIGEN; ANTIGENICITY; bacterial; capsular polysaccharide; Carbohydrate Conformation; Carbohydrate Sequence; Carbon; Chromatography; Chromatography,Ion Exchange; CONFIGURATION; conformation; COUPLING; Fourier Analysis; Hydrolysis; immunology; isolation & purification; MAGNETIC; Magnetic Resonance Spectroscopy; MAGNETIC-RESONANCE; Methods; Neisseria; Neisseria meningitidis; NMR; NMR analysis; NUCLEAR; Nuclear Magnetic Resonance; NUCLEAR-MAGNETIC-RESONANCE; ONE-BOND; one-dimensional; POLYSACCHARIDE; Polysaccharides; Polysaccharides,Bacterial; PROTON; RESONANCE; STRUCTURAL; structure; SUBSTITUENT; TWO-DIMENSIONAL NMR
AbstractThe capsular polysaccharide antigen of Neisseria meningitidis group I was isolated by Cetavlon precipitation and purified by ion-exchange chromatography. The structure of the I polysaccharide was determined largely by comprehensive proton and carbon-13 nuclear magnetic resonance studies in which both one-dimensional and two-dimensional experiments were carried out directly on the I polysaccharide. The I polysaccharide is composed of the repeating unit----4)alpha-L-GulpNAcA(1----3)[4-OAc]beta-D-ManpNA-cA(-- --in which the former residue adopts the 4C1 (L) conformation and the latter residue adopts the 4C1 (D) conformation. The one-bond coupling between the anomeric carbon and proton (1J13C,H) of the 2-acetamido-2-deoxy-beta-D-mannuronopyranosyl residue is not consistent with its beta-D configuration. This anomalous value of 1J13C,H for this residue is due to through-space anisotropy effects on its anomeric proton, generated by the proximity of the carboxyl group of the neighboring 2-acetamido-2-deoxy-alpha-L-guluronopyranosyl residue. The O-acetyl substituents of the I polysaccharide are essential for its antigenicity to group I polysaccharide-specific antibodies
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberMICHON1985B
NPARC number9358139
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Record identifier9c843270-13c1-466e-a0b4-4dbe19db654c
Record created2009-07-10
Record modified2016-05-09
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