In vivo determination of Neisseria meningitidis serogroup A capsular polysaccharide by whole cell high-resolution magic angle spinning NMR spectroscopy: Carbohydr.Res.

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TypeArticle
Journal titleCarbohydr.Res.
Volume341
Issue4
Pages557562; # of pages: 6
SubjectAcetylation; angle; Bacteria; capsular polysaccharide; carbohydrate; cell; CELL-SURFACE; complex; COMPLEXES; CPS; DISEASE; EXPRESSION; HIGH-RESOLUTION; Laboratories; magic angle; magic angle spinning; MUTANT; Neisseria; Neisseria meningitidis; NMR; NMR spectroscopy; NMR-spectroscopy; PATHOGENESIS; Polymers; POLYSACCHARIDE; SIGNALS; SPECTROSCOPY; STRAIN; structure; surface
AbstractHigh resolution-magic angle spinning (HRMAS) NMR spectroscopy was applied to serogroup A Neisseria meningitidis (NMA) to determine precise structures of capsular polysaccharide (CPS) expressed on the meningococcal surface. Both the O-acetylated (OAc) NMA parent and a mynC::aphA3 OAc- mutant demonstrated characteristic CPS-derived NMR signals indicating cell-surface expression of CPS, but only the parent expressed O-3 and O-4 acetylation signals. A capsule-defective strain showed no NMR signals for CPS. The (1)H NMR HRMAS spectral patterns correlated with the purified CPS (1)H NMR profiles. HRMAS NMR can distinguish detailed complex carbohydrate structures expressed on bacteria. NMA express both O-3 and O-4 acetylated polymers but not in equimolar ratio amounts in vivo
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberGUDLAVALLETI2006
NPARC number9358579
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Record identifier9cf85dbe-f228-4eb6-9922-8d393d1890f5
Record created2009-07-10
Record modified2016-05-09
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