Evidence of a role for S. cerevisiae α-arrestin Art1 (Ldb19) in mating projection and zygote formations

Download
  1. (PDF, 393 KB)
  2. Get@NRC: Evidence of a role for S. cerevisiae α-arrestin Art1 (Ldb19) in mating projection and zygote formations (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1002/cbin.10541
AuthorSearch for: ; Search for:
TypeArticle
Journal titleCell Biology International
ISSN1065-6995
Volume40
Issue1
Pages8390
AbstractThe discovery of arrestin-mediated biased signalling mechanisms for mammalian G-protein coupled receptors (GPCRs) has revolutionized the field over the last decade. Now, with the recent demonstration of a role for α-arrestins in internalization of the yeast pheromone GPCR, Ste2p, the possibility of arrestin-mediated alternate GPCR functionalities in yeast also follows. Here, the effects of knockout and complementation of yeast α-arrestin expression during mating are reported. Although minor effects on classical pheromone-related signalling are noted for a few arrestins, much stronger effects were observed downstream of cell cycle arrest, in particular linking Ldb19 (Art1) to mediation of zygote formation. Subsequent phenotypic observations linked this activity to more pronounced projection formation in an Art1 complemented noncuple α-arrestin knockout line, compared to the knockout-line alone, or either of the Art3 or Art6 complemented lines. Together with the observation of ligand-stimulated localization of Art-GFP to the mating projection, a possible role for this arrestin-like protein in projection formation is supported. While leaving the full mechanism of alternate Art1 functionality to be elucidated, together these findings implicate Art1 in selective regulation of mating events downstream of receptor internalization and cell cycle arrest, leading to schmoo, and ultimately zygote formation.
Publication date
PublisherWiley
LanguageEnglish
AffiliationAquatic and Crop Resource Development; National Research Council Canada
Peer reviewedYes
NPARC number23001871
Export citationExport as RIS
Report a correctionReport a correction
Record identifier9dcc437a-f913-412c-a182-835f6690785e
Record created2017-05-03
Record modified2017-05-03
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)