Spectroscopically encoded microspheres for antigen biosensing

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DOIResolve DOI: http://doi.org/10.1021/la700701x
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TypeArticle
Journal titleLangmuir
ISSN0743-7463
Volume23
Issue12
Pages64826485; # of pages: 4
AbstractHere we demonstrate the potential of barcoded resins (BCRs) as a reliable platform for immunoassays. Four BCRs were synthesized by dispersion polymerization of 4-methylstyrene, t-butylstyrene, 2,4-dimethylstyrene, and 2,5-dimethylstyrene. Methacrylic acid was included in the polymerization step to provide an anchor point for antibody conjugation. In addition to identifying the BCRs through their unique spectrum in an immunoassay experiment, Raman scattering spectroscopy confirmed the immunoreactivity of the bead-conjugated antibody by detecting 150 ng/mL (~150 pg/bead) of fluorescently labeled rabbit IgG antigen. The simplicity, versatility, and effectiveness of this platform demonstrate its potential for high-throughput multiplexed bioassays.
Publication date
PublisherAmerican Chemical Society
LanguageEnglish
AffiliationNational Research Council Canada; NRC Steacie Institute for Molecular Sciences; National Institute for Nanotechnology
Peer reviewedYes
Identifier10367391
NRC number576
NPARC number12327131
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Record identifier9df0b63c-98eb-41b9-aa96-d3e4dc2fc6d1
Record created2009-09-10
Record modified2017-03-17
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