Phase evolution in cholesterol/DPPC monolayers : atomic force microscopy and near field scanning optical microscopy studies

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DOIResolve DOI: http://doi.org/10.1046/j.0022-2720.2001.00982.x
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TypeArticle
Journal titleJournal of Microscopy
Volume205
Issue2
Pages136146; # of pages: 11
SubjectAFM; cholesterol; fluorescence; Langmuir-Blodgett
AbstractA combination of atomic force microscopy (AFM) and near field scanning optical microscopy has been used to study domain formation in dipalmitoylphosphatidylcholine (DPPC)/cholesterol monolayers with cholesterol concentrations ranging from 0 to 50%. The results show a clear evolution from a mixture of liquid expanded and liquid condensed phases for cholesterol concentrations?<?10% to a mixture of liquid expanded and two cholesterol-containing phases at intermediate concentrations, and finally to a single homogeneous liquid ordered phase for 33% cholesterol. Mixtures of the various phases are clearly identified by height differences in AFM and in some cases by fluorescence imaging for samples containing 0.5% BODIPY dye, which localizes preferentially in the more fluid phase. Note that fluorescence imaging, at least with the dye used here, is unable to distinguish between the cholesterol-rich and cholesterol-poor phases detected at intermediate cholesterol concentrations. The combination of fluorescence and AFM imaging provides a more complete picture of the phase evolution for cholesterol/DPPC monolayers than could be obtained by either technique alone, and presents substantial advantages over conventional fluorescence microscopy in that submicrometre-sized domains can be readily detected.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Steacie Institute for Molecular Sciences
Peer reviewedNo
NPARC number12338911
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Record identifiera1941323-791d-44ce-8d5d-db8135acc039
Record created2009-09-11
Record modified2016-05-09
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