A mussel (Mytilus edulis) tissue certified reference material for the marine biotoxins azaspiracids

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DOIResolve DOI: http://doi.org/10.1007/s00216-014-8250-5
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TypeArticle
Journal titleAnalytical and Bioanalytical Chemistry
ISSN1618-2642
Volume407
Issue11
Pages29852996; # of pages: 12
SubjectAzaspiracids; Matrix certified reference material; Seafood safety; Matrix effects; Liquid chromatography; mass spectrometry
AbstractAzaspiracids (AZAs) are lipophilic biotoxins produced by marine algae that can contaminate shellfish and cause human illness. The European Union (EU) regulates the level of AZAs in shellfish destined for the commercial market, with liquid chromatography-mass spectrometry (LC-MS) being used as the official reference method for regulatory analysis. Certified reference materials (CRMs) are essential tools for the development, validation, and quality control of LC-MS methods. This paper describes the work that went into the planning, preparation, characterization, and certification of CRM-AZA-Mus, a tissue matrix CRM, which was prepared as a wet homogenate from mussels (Mytilus edulis) naturally contaminated with AZAs. The homogeneity and stability of CRM-AZA-Mus were evaluated, and the CRM was found to be fit for purpose. Extraction and LC-MS/MS methods were developed to accurately certify the concentrations of AZA1 (1.16 mg/kg), AZA2 (0.27 mg/kg), and AZA3 (0.21 mg/kg) in the CRM. Quantitation methods based on standard addition and matrix-matched calibration were used to compensate for the matrix effects in LC-MS/MS. Other toxins present in this CRM at lower levels were also measured with information values reported for okadaic acid, dinophysistoxin-2, yessotoxin, and several spirolides.]
Publication date
LanguageEnglish
AffiliationMeasurement Science and Standards; National Research Council Canada
Peer reviewedYes
NPARC number21272755
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Record identifiera2c96d5f-56b6-4748-abcc-85bf868c33f6
Record created2014-12-03
Record modified2016-05-09
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