Direct Measurement of the Full, Sequence-Dependent Folding Landscape of a Nucleic Acid

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DOIResolve DOI: http://doi.org/10.1126/science.1133601
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TypeArticle
Journal titleScience
Volume314
Issue5801
Pages10011004; # of pages: 4
AbstractNucleic acid hairpins provide a powerful model system for understanding macromolecular folding, with free-energy landscapes that can be readily manipulated by changing the hairpin sequence. The full shapes of energy landscapes for the reversible folding of DNA hairpins under controlled loads exerted by an optical force clamp were obtained by deconvolution from high-resolution, single-molecule trajectories. The locations and heights of the energy barriers for hairpin folding could be tuned by adjusting the number and location of G:C base pairs, and the presence and position of folding intermediates were controlled by introducing single-nucleotide mismatches.
Publication date
AffiliationNational Research Council Canada; National Institute for Nanotechnology
Peer reviewedNo
NPARC number12339114
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Record identifiera37349a6-2d89-4c64-a96e-81bbb84f5956
Record created2009-09-11
Record modified2016-05-09
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