HVOF-sprayed nano TiO₂-HA coatings exhibiting enhanced biocompatibility

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DOIResolve DOI: http://doi.org/10.1007/s11666-009-9442-y
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
Journal titleJournal of Thermal Spray Technology
Pages336343; # of pages: 8
SubjectBiocompatibility; biomedical coating; human mesenchymal stem cells (hMSCs); HVOF spraying; implants; nanostructured TiO₂
AbstractBiomedical thermal spray coatings produced via high-velocity oxy-fuel (HVOF) from nanostructured titania (n-TiO₂) and 10 wt.% hydroxyapatite (HA) (n-TiO₂-10wt.%HA) powders have been engineered as possible future alternatives to HA coatings deposited via air plasma spray (APS). This approach was chosen due to (i) the stability of TiO2 in the human body (i.e., no dissolution) and (ii) bond strength values on Ti-6Al-4V substrates more than two times higher than those of APS HA coatings. To explore the bioperformance of these novel materials and coatings, human mesenchymal stem cells (hMSCs) were cultured from 1 to 21 days on the surface of HVOF-sprayed n-TiO₂ and n-TiO₂-10 wt.%HA coatings. APS HA coatings and uncoated Ti-6Al-4V substrates were employed as controls. The profiles of the hMSCs were evaluated for (i) cellular proliferation, (ii) biochemical analysis of alkaline phosphatase (ALP) activity, (iii) cytoskeleton organization (fluorescent/confocal microscopy), and (iv) cell/substrate interaction via scanning electron microscopy (SEM). The biochemical analysis indicated that the hMSCs cultured on n-TiO₂-10 wt.%HA coatings exhibited superior levels of bioactivity than hMSCs cultured on APS HA and pure n-TiO2 coatings. The cytoskeleton organization demonstrated a higher degree of cellular proliferation on the HVOF-sprayed n-TiO₂-10wt.%HA coatings when compared to the control coatings. These results are considered promising for engineering improved performance in the next generation of thermally sprayed biomedical coatings.
Publication date
AffiliationNRC Industrial Materials Institute; National Research Council Canada
Peer reviewedNo
NRC number52408
NPARC number18398984
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Record identifiera39c67ce-f097-4211-bea7-784c8ad76161
Record created2011-08-17
Record modified2016-05-09
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