Development of osteoblast colonies on new bioactive coatings

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Journal titleJournal of Thermal Spray Technology
Pages628633; # of pages: 6
Subjectbioactive coatings; hydroxyapatite; nano titania; osteoblast adhesion; polymer composite substrate; Ti-6Al-4V substrate
AbstractThe aging baby boomer population coupled with an increase in life expectancy is leading to a rising number of active elderly persons in occidental countries. As a result, the orthopedic implant industry is facing numerous challenges such as the need to extend implant life, reduce the incidence of revision surgery, and improve implant performance. This paper reports results of an investigation on the bioperformance of newly developed coating-substrate systems. Hydroxyapatite (HA) and nano-titania (nano-TiO₂) coatings were produced on Ti-6Al-4V and fiber reinforced polymer composite substrates. In vitro studies were conducted to determine the capacity of bioactive coatings developed to sustain osteoblast cells (fetal rat calvaria) adherence, growth, and differentiation. As revealed by scanning electron microscopy (SEM) observations and alkaline phosphatase activity, cell adhesion and proliferation demonstrated that HA coatings over a polymer composite are at least as good as HAcoatings made over Ti-6Al-4V substrate in terms of osteoblast cell activity. Nano-TiO₂ coatings produced by high-velocity oxyfuel (HVOF) spraying led to different results. For short-term cell culture (4.5 and 24 h), the osteoblasts appeared more flattened when grown on nano-TiO₂ than on HA. The surface cell coverage after seven days of incubation was also more complete on nano-TiO₂ than HA. Preliminary results indicate that osteoblast activity after 15 days of incubation on nano-TiO₂ is equivalent to or greater than that observed on HA.
Publication date
AffiliationNational Research Council Canada (NRC-CNRC); NRC Industrial Materials Institute; NRC Institute for Biological Sciences
Peer reviewedYes
NRC number48874
NPARC number15884119
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Record identifiera3d6efcb-bbd8-449e-a417-0314eb8c0543
Record created2010-07-30
Record modified2016-05-09
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