Separation of rare oligodendrocyte progenitor cells from brain using a high-throughput multilayer thermoplastic-based microfluidic device

  1. Get@NRC: Separation of rare oligodendrocyte progenitor cells from brain using a high-throughput multilayer thermoplastic-based microfluidic device (Opens in a new window)
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Journal titleBiomaterials
Pages55885593; # of pages: 6
SubjectCell sorting; Central nervous systems; Cutting edge technology; Membrane pore size; Micro-fluidic devices; Oligodendrocytes; Regenerative medicine; Separation efficiency; Cell culture; Fluidic devices; Multilayers; Reinforced plastics; Repair; Stem cells; Separation; myelin; animal cell; article; brain tissue; cell culture; cell differentiation; cell population; cell separation; cell size; cell viability; controlled study; flow rate; glia cell; in vitro study; microfluidics; nonhuman; oligodendrocyte progenitor cell; priority journal; rabbit; stem cell; Rattus
AbstractDespite the advances made in the field of regenerative medicine, the progress in cutting-edge technologies for separating target therapeutic cells are still at early stage of development. These cells are often rare, such as stem cells or progenitor cells that their overall properties should be maintained during the separation process for their subsequent application in regenerative medicine. This work, presents separation of oligodendrocyte progenitor cells (OPCs) from rat brain primary cultures using an integrated thermoplastic elastomeric (TPE)- based multilayer microfluidic device fabricated using hot-embossing technology. OPCs are frequently used in recovery, repair and regeneration of central nervous system after injuries. Indeed, their ability to differentiate invitro into myelinating oligodendrocytes, are extremely important for myelin repair. OPCs form 5-10% of the glial cells population. The traditional macroscale techniques for OPCs separation require pre-processing of cells and/or multiple time consuming steps with low efficiency leading very often to alteration of their properties. The proposed methodology implies to separate OPCs based on their smaller size compared to other cells from the brain tissue mixture. Using aforementioned microfluidic chip embedded with a 5μm membrane pore size and micropumping system, a separation efficiency more than 99% was achieved. This microchip was able to operate at flow rates up to 100μl/min, capable of separating OPCs from a confluent 75cm2 cell culture flask in less than 10min, which provides us with a high-throughput and highly efficient separation expected from any cell sorting techniques. © 2013 Elsevier Ltd.
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AffiliationNational Research Council Canada (NRC-CNRC)
Peer reviewedYes
NPARC number21270718
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Record identifiera464c901-09af-45ee-8b68-c7ecb3a996af
Record created2014-02-17
Record modified2016-05-09
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