Protective effect of ginsenosides Rg1 and Re on lipopolysaccharide-induced sepsis by competitive binding to toll-like receptor 4

Download
  1. Get@NRC: Protective effect of ginsenosides Rg1 and Re on lipopolysaccharide-induced sepsis by competitive binding to toll-like receptor 4 (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1128/AAC.01381-15
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleAntimicrobial Agents and Chemotherapy
ISSN0066-4804
Volume59
Issue9
Pages56545663; # of pages: 10
Subjectcyclooxygenase; ginsenoside Re; inducible nitric oxide synthase; interleukin; lipopolysaccharide; messenger RNA; protective agent; toll like receptor 4; tumor necrosis factor alpha; animal experiment; antisepsis; controlled study; drug; fever; gene; immune response; in vitro study; in vivo study; leukocyte count; macrophage; mouse; neutrophil count; nonhuman
AbstractWe previously demonstrated that ginsenosides Rg1 and Re enhanced the immune response in C3H/HeB mice but not in C3H/HeJ mice carrying a mutation in the Tlr4 gene. The results of the present study showed that both Rg1 and Re inhibited mRNA expression and production of proinflammatory mediators that included tumor necrosis factor a, interleukin-1β, interleukin-6, cyclooxygenase-2, and inducible nitric oxide synthase from lipopolysaccharide (LPS)-stimulated macrophages. Rg1 was found to be distributed both extracellularly and intracellularly but Re was located only extracellularly to compete with LPS for binding to Toll-like receptor 4. Preinjection of Rg1 and Re into rats suppressed LPS-induced increases in body temperature, white blood cell counts, and levels of serum proinflammatory mediators. Preinjection of Rg1 and Re into mice prevented the LPS-induced decreases in total white blood cell counts and neutrophil counts, inhibited excessive expression of multiple proinflammatory mediators, and successfully rescued 100% of the mice from sepsis-associated death. More significantly, when administered after lethal LPS inoculation, Rg1, but not Re, still showed a potent antisepsis effect and protected 90% of the mice from death. The better protection efficacy of Rg1 could result from its intracellular distribution, suggesting that Rg1 may be an ideal antisepsis agent.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); Human Health Therapeutics
Peer reviewedYes
NPARC number21276981
Export citationExport as RIS
Report a correctionReport a correction
Record identifiera4cf73b1-5d7e-4a4f-b1d0-1541d66d4039
Record created2015-11-10
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)