Transmission X-ray microscopy for full-field nano imaging of biomaterials

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DOIResolve DOI: http://doi.org/10.1002/jemt.20907
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TypeArticle
Journal titleMicroscopy Research and Technique
ISSN1059-910X
Volume74
Issue7
Pages671681; # of pages: 11
Subjectmercury; animal; bone; chemistry; cytology; equipment design; human; intracellular space; methodology; microscopy; mouse; nanotechnology; phase contrast microscopy; plant root; review; Schizosaccharomyces; spectrometry; tomography; X ray; Animals; Bone and Bones; Equipment Design; Humans; Intracellular Space; Mercury; Mice; Microscopy; Microscopy, Phase-Contrast; Nanotechnology; Plant Roots; Schizosaccharomyces; Spectrometry, X-Ray Emission; Tomography, X-Ray; X-Rays
AbstractImaging of cellular structure and extended tissue in biological materials requires nanometer resolution and good sample penetration, which can be provided by current full-field transmission X-ray microscopic techniques in the soft and hard X-ray regions. The various capabilities of full-field transmission X-ray microscopy (TXM) include 3D tomography, Zernike phase contrast, quantification of absorption, and chemical identification via X-ray fluorescence and X-ray absorption near edge structure imaging. These techniques are discussed and compared in light of results from the imaging of biological materials including microorganisms, bone and mineralized tissue, and plants, with a focus on hard X-ray TXM at ≤ 40-nm resolution. © 2010 Wiley-Liss, Inc.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for National Measurement Standards (INMS-IENM)
Peer reviewedYes
NPARC number21271319
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Record identifiera565902f-f667-4815-9682-16b2aaf7929c
Record created2014-03-24
Record modified2016-05-09
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