Structural characterization of the outer core and the O-chain linkage region of lipopolysaccharide from Pseudomonas aeruginosa serotype O5

Download
  1. Get@NRC: Structural characterization of the outer core and the O-chain linkage region of lipopolysaccharide from Pseudomonas aeruginosa serotype O5 (Opens in a new window)
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleEuropean Journal of Biochemistry
Volume267
Issue6
Pages16401650; # of pages: 11
SubjectALPHA; analysis; ANTIGEN; Antigens; bacterial; Bacterial Capsules; Canada; capsule; Carbohydrate Sequence; chemistry; classification; Comparative Study; COMPONENT; CONFIGURATION; core oligosaccharide; CORE REGION; LIPOPOLYSACCHARIDE; Lipopolysaccharides; LPS; Magnetic Resonance Spectroscopy; method; Methods; Molecular Sequence Data; MUTANT; NMR spectroscopy; NOESY; O Antigens; O-antigen; O-chain; oligosaccharide; Oligosaccharides; one-dimensional; POLYSACCHARIDE; Pseudomonas aeruginosa; receptor; REGION; REPEATING UNIT; RESIDUES; SEROTYPE; Serotyping; SMOOTH; SPECIFICITY; SPECTROSCOPY; Spectrum Analysis,Mass; STRAINS; STRUCTURAL CHARACTERIZATION; structure; Support,Non-U.S.Gov't; TOCSY; TRANSLOCATION; TRISACCHARIDE; TWO-DIMENSIONAL NMR; UNIT
AbstractThe point of attachment of the O-chain in the outer core region of Pseudomonas aeruginosa serotype O5 lipopolysaccharide (LPS) was determined following a detailed analysis of the extended core oligosaccharide, containing one trisaccharide O-chain repeating unit, present in both the wild-type strain PAO1 and O-chain deficient mutant strains AK1401 and PAO-rfc. The structure of the extended core oligosaccharide was determined by various mass spectrometric methods as well as one-dimensional and two-dimensional NMR spectroscopy. Furthermore, the one-dimensional analogues of NOESY and TOCSY experiments were applied to confirm the structure of the outer core region in the O-chain polysaccharide. In both the extended core oligosaccharide and the core of the smooth LPS, a loss of one of the beta-glucosyl residues and the translocation of the alpha-rhamnosyl residue, followed by the attachment of the first O-chain repeating unit was observed. This process is complicated and could involve two distinct rhamnosyltransferases, one with alpha-1, 6-linkage specificity and another with alpha-1,3-linkage specificity. It is also plausible that an alpha-1,3 rhamnosyltransferase facilitates the addition of the 'new' alpha-rhamnosyl residue that will act as a receptor for the attachment of the single O-antigen repeating unit in the LPS of the semi-rough mutant. The 2-amino-2-deoxy-fucosyl residue of the first O-chain repeating unit directly attached to the core was found to have a beta-anomeric configuration instead of an alpha configuration, characteristic for this residue as a component of the O-chain polysaccharide. The results of this study provide the first example of the mechanistic implications of the structure of the outer core region in a fully assembled O-chain containing LPS, differing from the O-chain deficient rough LPS
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedYes
NRC numberSADOVSKAYA2000
NPARC number9364720
Export citationExport as RIS
Report a correctionReport a correction
Record identifiera5b1764e-9384-4c6d-bf1f-ab21b338b0d6
Record created2009-07-10
Record modified2016-06-01
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)