Photonic wire biosensor microarray chip and instrumentation with application to serotyping of Escherichia coli isolates

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DOIResolve DOI: http://doi.org/10.1364/OE.21.004623
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TypeArticle
Journal titleOptics Express
ISSN1094-4087
Volume21
Issue4
Pages46234637; # of pages: 15
SubjectBiosensor microarrays; Chip layout; Coupling light; Delivery channels; E. coli; Fluid delivery; Fully compatible; Independent sensors; Molecular binding; Molecular biosensors; Optical alignments; Photonic wires; Polyclonal antibody; Probe molecules; Real time; Ring resonator; Sensor chips; Serotyping; Silicon photonic wires; Sub-wavelength; Surface grating couplers; Two-dimensional detectors; User input; Bioassay; Biochips; Escherichia coli; Instruments; Two dimensional; Sensors
AbstractA complete photonic wire molecular biosensor microarray chip architecture and supporting instrumentation is described. Chip layouts with 16 and 128 independent sensors have been fabricated and tested, where each sensor can provide an independent molecular binding curve. Each sensor is 50 μm in diameter, and consists of a millimeter long silicon photonic wire waveguide folded into a spiral ring resonator. An array of 128 sensors occupies a 2 × 2 mm2 area on a 6 × 9 mm2 chip. Microfluidic sample delivery channels are fabricated monolithically on the chip. The size and layout of the sensor array is fully compatible with commercial spotting tools designed to independently functionalize fluorescence based biochips. The sensor chips are interrogated using an instrument that delivers sample fluid to the chip and is capable of acquiring up to 128 optical sensor outputs simultaneously and in real time. Coupling light from the sensor chip is accomplished through arrays of sub-wavelength surface grating couplers, and the signals are collected by a fixed two-dimensional detector array. The chip and instrument are designed so that connection of the fluid delivery system and optical alignment are automated, and can be completed in a few seconds with no active user input. This microarray system is used to demonstrate a multiplexed assay for serotyping E. coli bacteria using serospecific polyclonal antibody probe molecules. © 2013 Optical Society of America.
Publication date
LanguageEnglish
AffiliationNRC Institute for Microstructural Sciences; Human Health Therapeutics; Measurement Science and Standards; Security and Disruptive Technologies; National Research Council Canada
Peer reviewedYes
NPARC number21270711
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Record identifiera5b384ae-2e0c-4d41-8b8a-3027d39cc7ea
Record created2014-02-17
Record modified2016-05-09
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