A common pathway for O-linked protein-glycosylation and synthesis of capsule in Acinetobacter baumannii

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DOIResolve DOI: http://doi.org/10.1111/mmi.12300
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TypeArticle
Journal titleMolecular Microbiology
ISSN0950-382X
Volume89
Issue5
Pages816830; # of pages: 15
Subjectglycoprotein; glycosyltransferase; pentasaccharide; Acinetobacter baumannii; article; bacterial capsule; bacterial virulence; biosynthesis; capsule biosynthesis; gene locus; glycoprotein synthesis; mass spectrometry; microbial attenuation; mouse; mutagenesis; nonhuman; nuclear magnetic resonance; priority journal; protein analysis; protein assembly; protein glycosylation; protein polymerization; protein structure; protein transport; reversed phase liquid chromatography; Acinetobacter baumannii; Bacteria (microorganisms); Mus
AbstractMulti-drug resistant strains of Acinetobacter baumannii are increasingly being isolated in hospitals worldwide. Among the virulence factors identified in this bacterium there is a general O-glycosylation system that appears to be important for biofilm formation and virulence, and the capsular polysaccharide, which is essential for resistance to complement killing. In this work, we identified a locus that is responsible for the synthesis of the O-pentasaccharide found on the glycoproteins. Besides the enzymes required for the assembly of the glycan, additional proteins typically involved in polymerization and transport of capsule were identified within or adjacently to the locus. Mutagenesis of PglC, the initiating glycosyltransferase prevented the synthesis of both glycoproteins and capsule, resulting in abnormal biofilm structures and attenuated virulence in mice. These results, together with the structural analysis of A. baumannii 17978 capsular polysaccharide via NMR, demonstrated that the pentasaccharides that decorate the glycoproteins are also the building blocks for capsule biosynthesis. Two linked subunits, but not longer glycan chains, were detected on proteins via MS. The discovery of a bifurcated pathway for O-glycosylation and capsule synthesis not only provides insight into the biology of A. baumannii but also identifies potential novel candidates for intervention against this emerging pathogen. © 2013 John Wiley & Sons Ltd.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences (IBS-ISB)
Peer reviewedYes
NPARC number21269804
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Record identifiera62261a8-857f-4a76-826f-810b35930d86
Record created2013-12-13
Record modified2016-05-09
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