Higher efficacy of dietary DHA provided as a phospholipid than as a triglyceride for brain DHA accretion in neonatal piglets

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DOIResolve DOI: http://doi.org/10.1194/jlr.M045930
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TypeArticle
Journal titleJournal of Lipid Research
ISSN1539-7262
Volume55
Issue3
Pages531539; # of pages: 9
Subjectdocosahexaenoic acid; phosphatidylcholine; phospholipid; triacylglycerol; animal experiment; animal tissue; article; body weight; brain cortex; controlled study; diet supplementation; erythrocyte; female; gray matter; liver; male; newborn; nonhuman; piglet; priority journal; retina; synaptosome; tissue distribution
AbstractLong-chain PUFAs (LCPUFAs) occur in foods primarily in the natural lipid classes, triacylglycerols (TAGs) or phospholipids (PLs). We studied the relative efficacy of the neural omega-3 DHA provided in formula to growing piglets as a dose of 13C-DHA bound to either TAG or phosphatidylcholine (PC). Piglets were assigned to identical formula-based diets from early life and provided with TAG-13C-DHA or PC-13C-DHA orally at 16 days. Days later, piglet organs were analyzed for 13C-DHA and other FA metabolites. PC-13C-DHA was 1.9-fold more efficacious for brain gray matter DHA accretion than TAG-13C-DHA, and was similarly more efficacious in gray matter synaptosomes, retina, liver, and red blood cells (RBCs). Liver labeling was greatest, implying initial processing in that organ followed by export to other organs, and suggesting that transfer from gut to bloodstream to liver in part drove the difference in relative efficacy for tissue accretion. Apparent retroconversion to 22:5n-3 was more than double for PC-13C-DHA and was more prominent in neural tissue than in liver or RBCs. These data directly support greater efficacy for PC as a carrier for LCPUFAs compared with TAG, consistent with previous studies of arachidonic acid and DHA measured in other species. Copyright © 2014 by the American Society for Biochemistry and Molecular Biology, Inc.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); Measurement Science and Standards (MSS-SME)
Peer reviewedYes
NPARC number21272243
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Record identifiera6ffc6cf-9fe4-49d9-89d5-c6f093c57448
Record created2014-07-23
Record modified2016-05-09
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