Sensitive determination of domoic acid in mussel tissue using dansyl chloride derivatization and liquid chromatography-mass spectrometry

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DOIResolve DOI: http://doi.org/10.1039/c4ay01853b
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TypeArticle
Journal titleAnalytical methods
ISSN1759-9660
AbstractThis paper describes a new method for sensitive determination of domoic acid (DA), the causative toxin of amnesic shellfish poisoning (ASP), in shellfish. The method involves extraction of tissue homogenates with 50% methanol followed by a highly selective strong anion exchange solid phase extraction and a derivatization with dansyl chloride (DNS-Cl) to form the dansyl derivative of domoic acid (DNS-DA). Reaction times were very rapid and proceeded under ambient conditions to yield stable derivatives. A study of the collision-induced dissociation of ESI-produced protonated DNS-DA was carried out to identify the most sensitive transitions to use in development of a selected reaction monitoring detection method. Compared with un-derivatized DA, DNS-DA showed a 5-fold increase in sensitivity of MS/MS detection and improved retention on a reversed phase LC stationary phase. Resolution of DNS-DA and its isomers was achieved using isocratic elution in 15 min.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; Measurement Science and Standards
Peer reviewedYes
NPARC number21274935
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Record identifiera8711077-7874-44ce-bc0a-5c6692b4fbf2
Record created2015-05-01
Record modified2016-10-27
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