Analysis of cyanobacterial toxins by hydrophilic interaction liquid chromatography-mass spectrometry

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DOIResolve DOI: http://doi.org/10.1016/j.chroma.2003.11.083
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TypeArticle
Journal titleJournal of Chromatography A
Volume1028
Issue1
Pages155164; # of pages: 10
Subjecthydrophilic interaction chromatography; bacteria; toxins; cyanotoxins; saxitoxins; cylindrospermopsin; anatoxins; microcystins
AbstractThe combination of hydrophilic interaction liquid chromatography with electrospray mass spectrometry (HILIC–MS) has been investigated as a tool for the analysis of assorted toxins produced by cyanobacteria. Toxins examined included saxitoxin and its various analogues (1–18), anatoxin-a (ATX-a, 19), cylindrospermopsin (CYN, 20), deoxycylindrospermopsin (doCYN, 21), and microcystins-LR (22) and -RR (23). The saxitoxins could be unequivocally detected in one isocratic analysis using a TSK gel Amide-80 column eluted with 65% B, where eluent A is water and B is a 95% acetonitrile/water solution, both containing 2.0 mM ammonium formate and 3.6 mM formic acid. The analysis of ATX-a, CYN and doCYN required 75% B isocratic. Simultaneous determination of 1–21 was also possible by using gradient elution. HILIC proved to be suitable for the analysis of microcystins, but peak shape was not symmetric and it was concluded that these compounds are best analysed using existing reversed-phase methods. The HILIC–MS method was applied to the analysis of field and cultured samples of Anabaena circinalis and Cylindrospermopsis raciborskii. In general, the method proved quite robust with similar results obtained in two different laboratories using different instrumentation.
Publication date
PublisherElsevier
Copyright notice© 2004 Elsevier B.V. All rights reserved.
LanguageEnglish
AffiliationNRC Institute for Marine Biosciences; National Research Council Canada; Measurement Science and Standards
Peer reviewedYes
NRC number1355
NPARC number3538122
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Record identifiera9b8734a-a015-4eaa-a3ef-969b714e04c4
Record created2009-03-01
Record modified2016-05-09
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