Comparison of lipopolysaccharide structures of Bordetella pertussis clinical isolates from pre- and post-vaccine era

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DOIResolve DOI: http://doi.org/10.1016/j.carres.2013.05.002
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TypeArticle
Journal titleCarbohydrate Research
ISSN0008-6215
Volume378
Pages5662; # of pages: 7
SubjectBordetella pertussis; Clinical isolates; Evolution; Gram-negative bacteria; Lipopolysaccharides; MALDI-mass spectrometry; Physico-chemical analysis; Vaccine strains; Chemical analysis; Epitopes; Mass spectrometry; Monoclonal antibodies; Polysaccharides; Structure (composition); Vaccines; lipid A; lipopolysaccharide; pertussis vaccine; article; bacterial strain; bacterium isolate; Bordetella pertussis; carbohydrate analysis; mass spectrometry; molecular model; nonhuman; physical chemistry; polyacrylamide gel electrophoresis; priority journal; thin layer chromatography; Bordetella pertussis; Negibacteria
AbstractEndotoxins are lipopolysaccharides (LPS), and major constituents of the outer membrane of Gram-negative bacteria. Bordetella pertussis LPS were the only major antigens, of this agent of whooping-cough, that were not yet analyzed on isolates from the pre- and post-vaccination era. We compared here the LPS structures of four clinical isolates with that of the vaccine strain BP 1414. All physico-chemical analyses, including SDS-PAGE, TLC, and different MALDI mass spectrometry approaches were convergent. They helped demonstrating that, on the contrary to some other B. pertussis major antigens, no modification occurred in the dodecasaccharide core structure, as well as in the whole LPS molecules. These results are rendering these major antigens good potential vaccine components. Molecular modeling of this conserved LPS structure also confirmed the conclusions of previous experiments leading to the production of anti-LPS monoclonal antibodies and defining the main epitopes of these major antigens. © 2013 Elsevier Ltd.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC)
Peer reviewedYes
NPARC number21270565
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Record identifiera9c346d2-f034-4969-8781-276ab8ebf0a8
Record created2014-02-17
Record modified2016-05-09
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