Complete chemoenzymatic synthesis of the Forssman antigen using novel glycosyltransferases identified in Campylobacter jejuni and Pasteurella multocida

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DOIResolve DOI: http://doi.org/10.1093/glycob/cwn117
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TypeArticle
Journal titleGlycobiology
Volume19
Issue2
Pages153159; # of pages: 7
SubjectCampylobacter jejuni; Forssman antigen; glycosyltransferase; Pasteurella multocida
AbstractWe have identified an α1,4-galactosyltransferase (CgtD) and a β1,3-N-acetylgalactosaminyltransferase (CgtE) in the lipooligosaccharide (LOS) locus of Campylobacter jejuni LIO87. Strains that carry these genes may have the capability of synthesizing mimics of the P blood group antigens of the globoseries glycolipids. We have also identified an α1,3-N-acetylgalactosaminyltransferase (Pm1138) from Pasteurella multocida Pm70, which is involved in the synthesis of an LOS-bound Forssman antigen mimic and represents the only known bacterial glycosyltransferase with this specificity. The genes encoding the three enzymes were cloned and expressed in Escherichia coli as soluble recombinant proteins that can be used to chemoenzymatically synthesize the Forssman antigen, and its biosynthetic precursors, in high yields.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences
Peer reviewedYes
NPARC number15295300
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Record identifiera9c45c83-8be0-46a4-9b57-297e7e95911e
Record created2010-05-17
Record modified2016-05-09
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