Microfabricated electrolysis pump system for isolating rare cells in blood

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DOIResolve DOI: http://doi.org/10.1088/0960-1317/13/4/324
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TypeArticle
Journal titleJournal of Micromechanics and Microengineering
ISSN0960-1317
Volume13
Issue4
PagesS164-S170
AbstractAn integrated system for immunomagnetic separation of rare cells from blood is presented. A micromachined device was fabricated by bonding silicon die with etched structures to a glass cover plate on which electrodes are defined. Electrolytic generation of gas from 0.50 M KNO3 (aqueous) provided pumping actuation for a device that performed the capture and purification of rare cells spiked into a 7.5 ul reconstituted blood sample. The system consisted of two pumps, a sample and a wash buffer meander reservoir, and a main channel for magnetic field trapping of rare cells captured by antibody-coated magnetic beads. A maximum pumping rate of 1.4 +- 0.1 ul min[?]1 was obtained at a current of 180 uA, and the maximum blood sample volume delivered to the capture bed was 6.5-7 ul. The trapped cells could be washed with the buffer from the second pump and then delivered to the exit port of the chip after removing the magnetic field.
Publication date
AffiliationNational Research Council Canada; National Institute for Nanotechnology
Peer reviewedNo
Identifier10487487
NPARC number12339031
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Record identifiera9f78cb8-af9f-478c-a4d2-b0c8a2f7bca5
Record created2009-09-11
Record modified2016-05-09
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