Tailoring the surface of a gene delivery vector with carboxymethylated dextran: A systematic analysis

  1. (PDF, 1 MB)
  2. Get@NRC: Tailoring the surface of a gene delivery vector with carboxymethylated dextran: A systematic analysis (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1021/acs.biomac.5b00221
AuthorSearch for: ; Search for: ; Search for: ; Search for:
Journal titleBiomacromolecules
Pages16711681; # of pages: 11
SubjectDextran; Electrostatic coatings; Gene transfer; Genes; Physiological models; Physiology; Carboxymethylated dextran; Gene delivery vectors; Physiological environment; Polymeric nanocarriers; Positively charged; Reporter-gene expression; Gene expression; carboxymethyldextran; deoxyribonuclease; nanocarrier; plasma protein; plasmid DNA; polymer; adsorption; erythrocyte; gene expression; gene vector; human; human cell; nanoencapsulation; nonviral gene delivery system; particle size; reporter gene; sheep; surface property; target cell
AbstractPolymeric nanocarriers are attractive nonviral vectors for gene delivery purposes in vivo. For such applications, numerous physiological and subcellular bottlenecks have to be overcome. In that endeavor, each structural feature of nanocarriers can be optimized with respect to its corresponding challenges. Here, we focused on the interface between a model gene delivery nanocarrier and relevant constituents of the physiological environment. We screened a library of carboxymethylated dextrans (CMD) for the electrostatic coating of positively charged nanocarriers. We evaluated the jointed influence of the CMD molecular weight and charge density upon nanocarrier coating with respect to DNase, small ions, plasma proteins, red blood cells, and target cells. A total of 4 out of 26 CMD coated nanocarriers successfully passed every screening assay, but did not yield increased reporter gene expression in target cells compared to uncoated nanocarriers. The fine-tuning of CMD for nanocarrier coating yielded a relevant shortlist of candidates that will be further tested in vivo.
Publication date
PublisherACS Publications
AffiliationNational Research Council Canada (NRC-CNRC); Human Health Therapeutics
Peer reviewedYes
NPARC number21275803
Export citationExport as RIS
Report a correctionReport a correction
Record identifierab1c9b68-9dc8-474c-a7ca-0ed13201ac54
Record created2015-07-14
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)