Real-time monitoring of adherent Vero cell density and apoptosis in bioreactor processes

Download
  1. Get@NRC: Real-time monitoring of adherent Vero cell density and apoptosis in bioreactor processes (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1007/s10616-011-9421-2
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleCytotechnology
ISSN0920-9069
Volume64
Issue4
Pages429441; # of pages: 13
Subjectapoptosis; in situ monitoring; cell density; Multi-frequency permittivity; Adherent Vero cell
AbstractThis study proposes an easy to use in situ device, based on multi-frequency permittivity measurements, tomonitor the growth and death ofattached Vero cells cultivated on microporous microcarriers, without any cell sampling. Vero cell densities were on-line quantified up to 10 6 cell mL -1. Some parameters which could potentially impact Vero cell morphological and physiological states were assessed through different culture operating conditions, such as media formulation or medium feed-harvest during cell growth phase. A new methodofinsitu cell death detection with dielectric spectroscopy was also successfully implemented. Thus, through permittivity frequency scanning, major rises of the apoptotic cell population in bioreactor cultures were detected by monitoring the characteristic frequency of the cell population, f c, which is one of the culture dielectric parameters. Both cell density quantification and cell apoptosis detection are strategic information in cell-based production processes as they are involved in major events of the process, suchasscale-uporchoice of the viral infection conditions. This new application of dielectric spectroscopy to adherent cell culture processes makes it a very promising tool for risk-mitigation strategy in industrial processes. Therefore, our results contribute to the development of Process Analytical Technology in cell-based industrial processes.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); Human Health Therapeutics
Peer reviewedYes
NPARC number21270219
Export citationExport as RIS
Report a correctionReport a correction
Record identifierab722eba-398b-44e3-8fe4-926f1fbabe0c
Record created2014-01-13
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)