Real-time monitoring, diagnosis, and time-course analysis of microalgae Scenedesmus AMDD cultivation using dual excitation wavelength fluorometry

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DOIResolve DOI: http://doi.org/10.1007/s10811-014-0494-0
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TypeArticle
Journal titleJournal of Applied Phycology
ISSN0921-8971
AbstractDual excitation wavelength fluorometry was used for real-time monitoring and diagnosis of the growth of the microalgae Scenedesmus AMDD in a 300-L continuous photobioreactor (PBR). Emission spectra were acquired at 1-min intervals using excitation lights at 365 and 540 nm. Real-time dry weight estimations were achieved using linear regression with the chlorophyll peak, while protein estimations required a more complex Principal Component Regression model, which takes advantage of the full emission spectrum. The resulting regression coefficients were 0.95 and 0.80, respectively. Furthermore, the spectra were analyzed using multivariate curve resolution technique. The proposed approach for fluorescence-based, real-time measurements of key algae cultivation parameters and culture state diagnosis was successfully demonstrated in a 42-day PBR validation test.
Publication date
PublisherSpringer International Publishing
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); Measurement Science and Standards; Aquatic and Crop Resource Development; Energy, Mining and Environment
Peer reviewedYes
NPARC number21275615
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Record identifierab8d3d55-c320-4463-833c-0e066413f9c3
Record created2015-07-14
Record modified2016-05-09
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