Aerobic biodegradation of N-nitrosodimethylamine by the propanotroph Rhodococcus ruber ENV425

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DOIResolve DOI: http://doi.org/10.1128/AEM.00418-09
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TypeArticle
Journal titleApplied and Environmental Microbiology
Volume75
Issue15
Pages50885093; # of pages: 6
SubjectENV
AbstractThe propanotroph Rhodococcus ruber ENV425 was observed to rapidly biodegrade N-nitrosodimethylamine (NDMA) after growth on propane, tryptic soy broth, or glucose. The key degradation intermediates were methylamine, nitric oxide, nitrite, nitrate, and formate. Small quantities of formaldehyde and dimethylamine were also detected. A denitrosation reaction, initiated by hydrogen atom abstraction from one of, the two methyl groups, is hypothesized to result in the formation of n-methylformaldimine and nitric oxide, the former of which decomposes in water to methylamine and formaldehyde and the latter of which is then oxidized further to nitrite and then nitrate. Although the strain mineralized more than 60% of the carbon in [¹⁴C]NDMA to ¹⁴CO2, growth of strain ENV425 on NDMA as a sole carbon and energy source could not be confirmed. The bacterium was capable of utilizing NDMA, as well as the degradation intermediates methylamine and nitrate, as sources of nitrogen during growth on propane. In addition, ENV425 reduced environmentally relevant microgram/liter concentrations of NDMA to <2 ng/liter in batch cultures, suggesting that the bacterium may have applications for groundwater remediation.
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute (BRI-IRB); National Research Council Canada
Peer reviewedNo
NRC number49980
NPARC number12427923
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Record identifierae89899e-9f4a-4d0f-8dd6-c4bacd0040b7
Record created2009-10-26
Record modified2016-05-09
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