The ether lipids of Methanosarcina mazei and other Methanosarcina species, compared by fast atom bombardment mass spectrometry

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DOIResolve DOI: http://doi.org/10.1139/m94-133
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TypeArticle
Journal titleCanadian Journal of Microbiology
Volume40
Pages837843; # of pages: 7
AbstractThe total lipids of Methanosarcina mazei accounted for 4.0% of the cell dry mass, and 90% of these were polar lipids. Nearly all of the polar fraction consisted of diether (2,3-di-O-phytanyl-sn-glycerol) and 3-hydroxydiether analogs of phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, and phosphatidylethanolamine. Several highly fluorescent trace components in the lipid extracts were purified and partially characterized by mass spectrometry. Fast atom bombardment mass spectrometry of total lipid extracts provided data to establish a close relationship among the polar lipids present in Methanosarcina mazei, Methanosarcina thermophila, Methanosarcina acetivorans, Methanosarcina barkeri Fusaro, and Methanosarcina barkeri MS.Key words: diether lipids, hydroxydiether lipids, fast atom bombardment mass spectrometry, Methanosarcina, archaeobacteria.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Institute for Biological Sciences
Peer reviewedNo
NRC numberSPROTT1994
37410
NPARC number9360714
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Record identifieraecae2ef-1eaf-4684-8c45-7772deb18903
Record created2009-07-10
Record modified2016-05-09
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