Application of dynamic light scattering in protein crystallization

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DOIResolve DOI: http://doi.org/10.1002/0471140864.ps1710s61
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TypeArticle
Journal titleCurrent protocols in protein science
VolumeSupplement 61
Pages17.10.117.10.9
SubjectDynamic light scattering; Crystallization of proteins; Crystallography
AbstractSuccess in determining the three-dimensional structure of a macromolecule by X-ray diffraction methods depends critically on the ability to obtain well ordered crystals of the macromolecule in question. Predisposition to crystallization correlates with the homogeneity of the molecules in solution. Dynamic light scattering (DLS) is particularly well suited for evaluating protein homogeneity under multiple conditions and at concentrations commensurate with crystallization conditions. This unit presents a typical protocol for DLS measurements of a protein sample, and describes approaches to improve protein homogeneity in solution.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Biotechnology Research Institute
Peer reviewedYes
NRC number53125
NPARC number16512466
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Record identifieraf581c5e-1b59-4f19-8993-ebc196765e5d
Record created2010-12-14
Record modified2016-05-09
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