O-linked glycosylation leads to decreased thermal stability of interferon alpha 2b as measured by two orthogonal techniques

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DOIResolve DOI: http://doi.org/10.1007/s11095-011-0402-0
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titlePharmaceutical Research
Volume28
Issue7
Pages16611667; # of pages: 7
Subjectactivity; Animal; Antibodies; bio; Biotechnology; Calorimetry; Canada; Cell Adhesion; Cells; chemistry; Circular Dichroism; crystal structure; derivatives; differential scanning calorimetry; FiSH; genetics; Glycosylation; HEK293; Hemocyanin; Human; I; immunology; In Vitro; inhibitors; interferon; Kidney; Melanoma; methods; microbiology; Molecular Biology; Nanoparticles; Peptides; Protein; Proteins; Reference Standards; Scopus; secondary; serum; Temperature; therapeutic use; therapy; Thermodynamics; toxicity; Vitro
AbstractThe utility of porous metals for the integration of orthopaedic implants with host bone has been well established. Quantification of the tissue response to cementless implants is laborious and time consuming process requiring tissue processing, embedding, sectioning, polishing, imaging and image analysis. Micro-computed tomography (μCT) is a promising three dimensional (3D) imaging technique to quantify the tissue response to porous metals. However, the suitability and effectiveness of μCT for the quantification of bone ingrowth remains unknown. The purpose of this study was to evaluate and compare bone growth within porous titanium implants using both μCT and traditional hard-tissue histology techniques. Cylindrical implants were implanted in the distal femora and proximal tibiae of a rabbit. After 6 weeks, bone ingrowth was quantified and compared by μCT, light microscopy and backscattered electron microscopy. Quantification of bone volume and implant porosity as determined by μCT compared well with data obtained by traditional histology techniques. Analysis of the 3D dataset showed that bone was present in the pores connected with openings larger 9.4 μm. For pore openings greater than 28.2 μm, the size of the interconnection had little impact on the bone density within the porosity for the titanium foams.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
NRC number52780
NPARC number18335883
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Record identifierb02ade1f-f218-4e95-83eb-3c766eaf732e
Record created2011-07-28
Record modified2016-05-09
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