Expression of and secretion through the Aeromonas salmonicida type III secretion system

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DOIResolve DOI: http://doi.org/10.1099/mic.0.28485-0
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TypeArticle
Journal titleMicrobiology
ISSN1350-0872
1465-2080
Volume152
Issue5
Pages12751286
SubjectTTSS; type III secretion system; ACN; acetonitrile; 2-DE; 2-dimensional gel electrophoresis; OMP; outer-membrane protein
AbstractAeromonas salmonicida subsp. salmonicida is the aetiological agent of furunculosis, a disease of farmed and wild salmonids. The type III secretion system (TTSS) is one of the primary virulence factors in A. salmonicida. Using a combination of differential proteomic analysis and reverse transcriptase (RT)-PCR, it is shown that A. salmonicida A449 induces the expression of TTSS proteins at 28 °C, but not at its more natural growth temperature of 17 °C. More modest increases in expression occur at 24 °C. This temperature-induced up-regulation of the TTSS in A. salmonicida A449 occurs within 30 min of a growth temperature increase from 16 to 28 °C. Growth conditions such as low-iron, low pH, low calcium, growth within the peritoneal cavity of salmon and growth to high cell densities do not induce the expression of the TTSS in A. salmonicida A449. The only other known growth condition that induces expression of the TTSS is growth of the bacterium at 16 °C in salt concentrations ranging from 0·19 to 0·38 M NaCl. It is also shown that growth at 28 °C followed by exposure to low calcium results in the secretion of one of the TTSS effector proteins. This study presents a simple in vitro model for the expression of TTSS proteins in A. salmonicida.
Publication date
PublisherMicrobiology Society
LanguageEnglish
AffiliationNRC Institute for Marine Biosciences; National Research Council Canada
Peer reviewedYes
NPARC number23000933
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Record identifierb10bd808-06fd-4e8a-aa23-62259e27c1e3
Record created2016-11-18
Record modified2016-11-18
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